Special antigens that you might need for antibody discovery
This process includes a literature search on the protein that drives the strategy design. The antigens are then produced in mammalian expression systems (EXpiCHOTM, ExpiHEKTM) and purified using affinity and size exclusion chromatography methods. QC is done via reducing and non-reducing SDS-PAGE, HPLC-SEC and relevant functional assays such us binding to ligand or to antibody control. In addition, the produced antigen can be biotinylated or conjugated to fluorochromes and QCed to be used further in phage and mammalian display campaigns.